Extraction and Characterization of Chitosan from the Shells of Indian White Prawn, Fenneropenaeus indicus (Edwards, 1837)
Keywords:
Acid-base treatment, characterization, chitin, FTIR, SEM, shrimp shellAbstract
The shell wastes of Indian white prawn, Fenneropenaeus indicus, collected from markets and restaurants, were used as raw material for the extraction of chitin and chitosan. The yields of chitin and chitosan extracted from shrimp were 33.35 ± 2.62% and 29.45 ± 1.77%, respectively. The solubility and water-binding capacity of chitosan were 100% and 830.44 ± 128.36%, respectively. The solubility and water-binding capacity of the extracted chitosan are high enough that it can easily be incorporated into various applications. Moreover, the ash content of chitosan produced was relatively low (0.1 ± 0.03%) from Fenneropenaeus indicus, which is indicative of effective demineralization and low impurities in the extracted chitosan. In addition, the functional groups of the chitosan and its surface morphology were identified by Fourier Transform Infrared (FTIR) spectra and Scanning Electron Microscopy (SEM). The featured peaks of the FTIR spectra of the extracted chitosan in this study were comparable to those in other related studies. Furthermore, the SEM micrographs of the surface morphology of chitosan revealed rough and fibrous structures with regular or irregular pores.
References
Ahing, F.A., Wid, N., 2016. Extraction and characterization of chitosan from shrimp shell waste in Sabah. Transactions on Science and Technology 3 (1-2), 227-237.
Aranaz, I., Alcántara, A.R., Civera, M.C., Arias, C., Elorza, B., Heras Caballero, A., Acosta, N., 2021. Chitosan: an overview of its properties and applications. Polymers 13 (3256), 1-27.
Badawy, M.E.I., Rabea, E.I., 2011. A biopolymer chitosan and its derivatives as promising antimicrobial agents against plant pathogens and their applications in crop protection. International Journal of Carbohydrate Chemistry 460381. https://doi.org/10.1155/2011/460381.
Bedoi´c, R., Cosi´c, B., Dui´c, N., 2019. Technical potential and geographic distribution of agricultural residues, co-products and by-products in the European Union. Science of the Total Environment 686, 568-579.
Cai, W., Liu, C., Zhang, C., Ma, M., Rao, W., Li, W., He, K., Gao, M., 2018. Developing the ecological compensation criterion of industrial solid waste based on energy for sustainable development. Energy 157 (1), 940-948.
Chakraborty, M., Hasanuzzaman, M., Rahman, M., Khan, M.A.R., Bhowmik, P., Mahmud, N.U., Tanveer, M., Islam, T., 2020. Mechanism of plant growth promotion and disease suppression by chitosan biopolymer. Agriculture 10, 624. https://doi.org/10.3390/agriculture10120624.
Chandumpai, A., Singhpibulporn, N., Faroongsarng, D., Sornprasit, P., 2004. Preparation and physico-chemical characterization of chitin and chitosan from the pens of the squid species, Loligo lessoniana and Loligo formosana. Carbohydrate Polymer 58, 467-474.
Dago-Serry, Y., Maroulas, K.N., Tolkou, A.K., Kokkinos, N.C., Kyzas, G.Z., 2024. How the chitosan structure can affect the adsorption of pharmaceuticals from wastewaters: an overview. Carbohydrate Polymer Technologies and Applications 7, 100466. https://doi.org/10.1016/j.carpta.2024.100466.
Dash, M., Chiellini, F., Ottenbrite, R.M., Chiellini, E., 2011. Chitosan- a versatile semi-synthetic polymer in biomedical applications. Progress in Polymer Science 36, 981-1014.
Dutta, P., Kumari, A., Mahanta, M., 2022. Chitosan nanoparticle: synthesis, characterization, and use as plant health materials. In: B. Kumar (Ed.), Chitin and chitosan- isolation, properties, and applications. IntechOpen: pp. 1-17.
Gadgey, K.K., Bahekar, A., 2017. Studies on extraction methods of chitin from crab shell and investigation of its mechanical properties. International Journal of Mechanical Engineering and Technology 8 (2), 220-231.
Gupta, K.C., Ravi Kumar, M.N.V., 2000. An overview on chitin and chitosan applications with an emphasis on controlled drug release formulations. Journal of Macromolecular Science, Part C: Polymer Reviews 40 (4), 273-308.
Ji, Y., Yang, X., Ji, Z., Zhu, L., Ma, N., Chen, D., Jia, X., Tang, J., Cao, Y., 2020. DFT-calculated IR spectrum amide I, II, and III band contributions of N-methylacetamide fine components. ACS Omega 5, 8572-8578.
Kumari, S., Annamareddy, K.S.H., Abanti, P., Rath, K., 2017. Physicochemical properties and characterization of chitosan synthesized from fish scales, crab and shrimp shells. International Journal of Biological Macromolecules 104, 1697-1705.
Milne Edwards, H., 1837. Histoire naturelle des crustacés, comprenant l´anatomie, la physiologie et la classification de ces animaux. Paris, Librairie encyclopédique de Roret. 560 pp.
Mukhtar, M., Fényes, E., Bartos, C., Zeeshan, M., Ambrus, R., 2021. Chitosan biopolymer, its derivatives and potential applications in nano-therapeutics: a comprehensive review. European Polymer Journal 160, 110767.
Myanmar Digital News, 2023. Myanmar earns US $69.425 million from the export of marine products in April, May 2023. https://www.mdn.gov.mm.
No, H.K., Meyers, S.P., 1995. Preparation and characterization of chitin and chitosan: a review. Journal of Aquatic Food Product Technology 4 (2), 27-52.
Orzali, L., Corsi, B., Forni, C., Riccioni, L., 2017. Chitosan in agriculture: A new challenge for managing plant disease. In: E. A. Shalaby (Ed.), Biological activities and application of marine polysaccharides. IntechOpen: pp. 87-96.
Puvvada, Y.S., Vankayalapati, S., Sukhavasi, S., 2012. Extraction of chitin from chitosan from exoskeleton of shrimp for application in the pharmaceutical industry. International Current Pharamaceutical Journal 1 (9), 258-263.
Pǎdurețu, C.C., Apetroaei, M.R., Rǎu, I., Schroder, V., 2018. Characterization of chitosan extracted from different Romanian black sea crustaceans. Chemistry and Materials Science 80 (3), 13-24.
Paul, S., Jayan, A., Sasikumar, C.S., Cherian, S.M., 2014. Extraction and purification of chitosan from chitin isolated from sea prawn (Fenneropenaeus indicus). Asian Journal of Pharmaceutical and Clinical Research 7 (4), 201-204.
Rege, P.R., Shukla, D.J., Block, L.H., 1999. Chitinosans as tableting excipients for modified release delivery systems. International Journal of Pharmaceutics 181 (1), 49-60.
Šimat, V., Rathod, N.B., Čagalj, M., Hamed, I., Mekinić, I.G. 2022. Astaxanthin from crustaceans and their byproducts: a bioactive metabolite candidate for therapeutic application. Marine Drugs 20 (3), 1-22.
Song, C., Yu, H., Zhang, M., Yang, Y., Zhang, G., 2013. Physicochemical properties and antioxidant activity of chitosan from the blowfly Chrysomya megacephala larvae. International Journal of Biological Macromolecules 60, 347-354.
Vino, A.B., Ramasamy, P., Shanmugam, V., Shanmugam, A., 2012. Extraction, characterization and in vitro antioxidative potential of chitosan and sulfated chitosan from cuttlebone of Sepia aculeata Orbigny, 1848. Asian Pacific Journal of Tropical Biomedicine 2, 334-341.
Wang, S.L., Nguyen, V.B., 2019. Production of potent antidiabetic compounds from shrimp head powder via Paenibacillus conversion. Process Chemistry 76, 18-24.
William, W., Wid, N., 2019. Comparison of extraction sequence on yield and physico-chemical characteristics of chitosan from shrimp shell waste. Journal of Physics. Conference Series 1358, 1-7.
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